Dnabinding domain of a versatile class of eukaryo tic transcription factors zinc finger proteins zfps. Genome editing with engineered nucleases in plants oxford. High frequency modification of plant genes using engineered zinc finger nucleases. Given these considerations, fully realizing the promise of therapeutic genome engineering will require a high degree of targeting precision, i. Pdf high frequency modification of plant genes using. Pdf genome editing with engineered nucleases in plants. Genome engineering with zincfinger nucleases genetics.
From mega nucleases, to zinc fingers, to talens, and now crisprcas, the use of engineered nucleases for genome editing has been gathering pace. Many knockoutknockin mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease zfn, transcription activatorlike effector nuclease talen, or clustered regularly interspaced short palindromic repeats crisprcas9. Even for mouse genomic engineering, zfns have several. Current methods of an engineered zfn design, the important use in. Zinc fingers are shown as open boxes, with short vertical lines indicating the main contacts with the. Genome editing with engineered zinc finger nucleases. Genome editing in mouse and rat by electroporation. Zinc finger nucleases zfns are versatile tools for making precise modifications to genomes, and their use is now established in a range of model systems.
Designed nucleases for targeted genome editing lee 2016. This video outlines these four basic genome editing. Such genome editing is now established in human cells and a number of model organisms, thus. Engineered gene editing nucleases are now poised to change that. High frequency modification of plant genes using engineered zinc finger nucleases article pdf available in nature 4597245. The source data for this figure is available in the source data file. The dna binding domains of zinc finger zf proteins were the first to be used as genome editing tools, in the form of designed zf nucleases zfns. Zinc finger nucleases as tools to understand and treat human. Diversifying the structure of zinc finger nucleases for.
Genome editing encompasses a wide variety of tools using either. The past and future of in vivo genome editing kaitlin vanderweide advised by dr. Color online schematics summarizing the mechanisms of gene editing nucleases. Methods for design, testing and implementation of zinc finger proteins zfps. The dnabinding do mains of zinc finger zf proteins were the first to be used as genome editing tools, in the form of designed zf nucleases. Various genetic engineering methodologies currently fall under the operative.